Coding

Part:BBa_K3747119

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Nitrous oxide reductase D (nosD) from Pseudomonas stutzeri JM300

This gene is part of the nos operon from P. stutzeri JM300. Nos is the last enzyme of the denitrification pathway, converting nitrous oxide (N2O) into dinitrogen gas (N2). NosD is a periplasmic interacting protein, which is presumably required for the shuttling of sulfur species to the periplasm for assembly of NosZ [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 491
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 127
    Illegal NgoMIV site found at 922
    Illegal AgeI site found at 658
    Illegal AgeI site found at 802
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Honisch, U., & Zumft, W. G. (2003). Operon Structure and Regulation of the nos Gene Region of Pseudomonas stutzeri, Encoding an ABC-Type ATPase for Maturation of Nitrous Oxide Reductase. Journal of Bacteriology, 185(6), 1895. https://doi.org/10.1128/JB.185.6.1895-1902.2003

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